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novus biosciences cat  (Novus Biologicals)


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    Novus Biologicals novus biosciences cat
    Novus Biosciences Cat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/novus+biosciences+cat/TLR4+Antibody+(76B357%2E1)+-+BSA+Free/pm41437986-66-29-29
    Average 93 stars, based on 59 article reviews
    novus biosciences cat - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Glial activation and increase in cerebral pro-inflammatory cytokine expression in a female animal post-COVID model.
    Article Snippet: For electrophoresis, 40 μg of protein from the brain was loaded onto a precast Mini-Protean® TGXTM gel (Bio-Rad, Spain) and transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, Spain). .. Membranes were blocked with 3 % non-fat dry milk at room temperature for 1h and then incubated at 4◦ overnight with the primary following antibodies: Toll-like receptor-4 (TLR4) 1:500 (Novus Biosciences Cat#NB100-56566); Myeloid differentiation primary response gene 88 (MyD88) 1:500 (Abcam, Cat#2064); Nuclear Factor kappa-light-chainenhancer of activated B cells (NF-κβ p65) 1:1000 (Abcam, Cat#16502); interleukins IL-6 1:500 (Abcam, Cat#290735); IL-1β 1:1000 (Abcam, Cat#283818); IL-18 1:750 (Invitrogen Cat#PA579482); integrin alpha D (CD11d) 1:1000 (Abcam, Cat#231534); glial fibrillary acidic proteins (GFAP) 2A5 1:1500 (Santa Cruz Biotechnology, Cat#sc-65343); ionized calcium-binding adapter molecule-1 (Iba1) 1:1000 (Abcam, Cat# 178846). .. After primary incubation, membranes were incubated for 1 h at room temperature with the specific secondary antibody (Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP (Thermo Fisher Scientific, Inc, Cat#31430) or goat anti-rabbit IgG (H + L) secondary antibody, AP (Thermo Fisher Scientific, Inc, Cat#31340).

    Article Title: Glial activation and increase in cerebral pro-inflammatory cytokine expression in a female animal post-COVID model
    Article Snippet: For electrophoresis, 40 μg of protein from the brain was loaded onto a precast Mini-Protean® TGXTM gel (Bio-Rad, Spain) and transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, Spain). .. Membranes were blocked with 3 % non-fat dry milk at room temperature for 1h and then incubated at 4° overnight with the primary following antibodies: Toll-like receptor-4 (TLR4) 1:500 (Novus Biosciences Cat#NB100-56566); Myeloid differentiation primary response gene 88 (MyD88) 1:500 (Abcam, Cat#2064); Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κβ p65) 1:1000 (Abcam, Cat#16502); interleukins IL-6 1:500 (Abcam, Cat#290735); IL-1β 1:1000 (Abcam, Cat#283818); IL-18 1:750 (Invitrogen Cat#PA5-79482); integrin alpha D (CD11d) 1:1000 (Abcam, Cat#231534); glial fibrillary acidic proteins (GFAP) 2A5 1:1500 (Santa Cruz Biotechnology, Cat#sc-65343); ionized calcium-binding adapter molecule-1 (Iba1) 1:1000 (Abcam, Cat# 178846). .. After primary incubation, membranes were incubated for 1 h at room temperature with the specific secondary antibody (Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP (Thermo Fisher Scientific, Inc, Cat#31430) or goat anti-rabbit IgG (H + L) secondary antibody, AP (Thermo Fisher Scientific, Inc, Cat#31340).



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    Representative immunoblots for <t>TLR4</t> (A), and MyD88 (B) protein expression in whole cardiac left ventricle. Diagram bars show the results of densitometric analysis in whole cardiac left ventricle. Data are presented as Mean ± SEM of observations obtained for 4–6 tissues samples from 4–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical.
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    Representative immunoblots for <t>TLR4</t> (A), and MyD88 (B) protein expression in whole cardiac left ventricle. Diagram bars show the results of densitometric analysis in whole cardiac left ventricle. Data are presented as Mean ± SEM of observations obtained for 4–6 tissues samples from 4–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical.
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    Image Search Results


    Representative immunoblots for TLR4 (A), and MyD88 (B) protein expression in whole cardiac left ventricle. Diagram bars show the results of densitometric analysis in whole cardiac left ventricle. Data are presented as Mean ± SEM of observations obtained for 4–6 tissues samples from 4–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical.

    Journal: Pharmacology Research & Perspectives

    Article Title: Cisplatin cycles treatment sustains cardiovascular and renal damage involving TLR4 and NLRP3 pathways

    doi: 10.1002/prp2.1108

    Figure Lengend Snippet: Representative immunoblots for TLR4 (A), and MyD88 (B) protein expression in whole cardiac left ventricle. Diagram bars show the results of densitometric analysis in whole cardiac left ventricle. Data are presented as Mean ± SEM of observations obtained for 4–6 tissues samples from 4–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical.

    Article Snippet: The membranes were blocked with 3% of non‐fat dry milk at room temperature for 1 h and then incubated at 4° overnight with the primary antibodies: TLR4 1:500 (left ventricle), 1:2000 (aorta), 1:1000 (kidney) (Novus Biosciences Cat#NB100‐56566); MyD88 1:500 (left ventricle, kidney), 1:1000 (aorta) (Abcam, Cat#2064); NLRP3 1:750 (left ventricle), 1:1000 (aorta, kidney) (Abcam, Cat#263899); procaspase‐1 1:1000 (left ventricle, aorta, kidney) (Abcam, Cat#286195); NF‐κβ p65 1:1000 (kidney) (Abcam, Cat#16502).

    Techniques: Western Blot, Expressing

    Representative immunoblots for TLR4 (A), and MyD88 (B) protein expression in aorta. Diagram bars show the results of densitometric analysis in aorta. Data are presented as Mean ± SEM of observations obtained for 4–6 tissues samples from 4–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical.

    Journal: Pharmacology Research & Perspectives

    Article Title: Cisplatin cycles treatment sustains cardiovascular and renal damage involving TLR4 and NLRP3 pathways

    doi: 10.1002/prp2.1108

    Figure Lengend Snippet: Representative immunoblots for TLR4 (A), and MyD88 (B) protein expression in aorta. Diagram bars show the results of densitometric analysis in aorta. Data are presented as Mean ± SEM of observations obtained for 4–6 tissues samples from 4–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical.

    Article Snippet: The membranes were blocked with 3% of non‐fat dry milk at room temperature for 1 h and then incubated at 4° overnight with the primary antibodies: TLR4 1:500 (left ventricle), 1:2000 (aorta), 1:1000 (kidney) (Novus Biosciences Cat#NB100‐56566); MyD88 1:500 (left ventricle, kidney), 1:1000 (aorta) (Abcam, Cat#2064); NLRP3 1:750 (left ventricle), 1:1000 (aorta, kidney) (Abcam, Cat#263899); procaspase‐1 1:1000 (left ventricle, aorta, kidney) (Abcam, Cat#286195); NF‐κβ p65 1:1000 (kidney) (Abcam, Cat#16502).

    Techniques: Western Blot, Expressing

    Representative images of immunohistochemistry (400×) of the mesenteric artery principal branches, after of cisplatin cycles administration. TLR4 expression in endothelial cells (red arrow) and smooth muscle cells (blue arrow) in cisplatin 2 mg/kg (A), and cisplatin 3 mg/kg (B) groups. MyD88 expression in endothelial cells (red arrow) and smooth muscle cells (blue arrow) in cisplatin 2 mg/kg (C), and cisplatin 3 mg/kg (D) groups. NLRP3 expression in endothelial cells (red arrow) and smooth muscle cells (blue arrow) in cisplatin 2 mg/kg (E), and cisplatin 3 mg/kg (F) groups.

    Journal: Pharmacology Research & Perspectives

    Article Title: Cisplatin cycles treatment sustains cardiovascular and renal damage involving TLR4 and NLRP3 pathways

    doi: 10.1002/prp2.1108

    Figure Lengend Snippet: Representative images of immunohistochemistry (400×) of the mesenteric artery principal branches, after of cisplatin cycles administration. TLR4 expression in endothelial cells (red arrow) and smooth muscle cells (blue arrow) in cisplatin 2 mg/kg (A), and cisplatin 3 mg/kg (B) groups. MyD88 expression in endothelial cells (red arrow) and smooth muscle cells (blue arrow) in cisplatin 2 mg/kg (C), and cisplatin 3 mg/kg (D) groups. NLRP3 expression in endothelial cells (red arrow) and smooth muscle cells (blue arrow) in cisplatin 2 mg/kg (E), and cisplatin 3 mg/kg (F) groups.

    Article Snippet: The membranes were blocked with 3% of non‐fat dry milk at room temperature for 1 h and then incubated at 4° overnight with the primary antibodies: TLR4 1:500 (left ventricle), 1:2000 (aorta), 1:1000 (kidney) (Novus Biosciences Cat#NB100‐56566); MyD88 1:500 (left ventricle, kidney), 1:1000 (aorta) (Abcam, Cat#2064); NLRP3 1:750 (left ventricle), 1:1000 (aorta, kidney) (Abcam, Cat#263899); procaspase‐1 1:1000 (left ventricle, aorta, kidney) (Abcam, Cat#286195); NF‐κβ p65 1:1000 (kidney) (Abcam, Cat#16502).

    Techniques: Immunohistochemistry, Expressing

    Representative immunoblots for TLR4 (A), MyD88 (B), and NF‐κβ p65 (C) protein expression in kidney. Diagram bars show the results of densitometric analysis in kidney. Data are presented as Mean ± SEM of observations obtained for 5–6 tissues samples from 5–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical (**** p < .0001 cisplatin 3 mg/kg vs. saline, *** p < .001 cisplatin 3 mg/kg vs. saline, ** p < .01 cisplatin 3 mg/kg vs. saline, * p < .05 cisplatin 3 mg/kg vs. saline, ### p < .001 cisplatin 3 mg/kg vs. cisplatin 2 mg/kg, ## p < .01 cisplatin 3 mg/kg vs. cisplatin 2 mg/kg, # p < .05 cisplatin 3 mg/kg vs. cisplatin 2 mg/kg).

    Journal: Pharmacology Research & Perspectives

    Article Title: Cisplatin cycles treatment sustains cardiovascular and renal damage involving TLR4 and NLRP3 pathways

    doi: 10.1002/prp2.1108

    Figure Lengend Snippet: Representative immunoblots for TLR4 (A), MyD88 (B), and NF‐κβ p65 (C) protein expression in kidney. Diagram bars show the results of densitometric analysis in kidney. Data are presented as Mean ± SEM of observations obtained for 5–6 tissues samples from 5–6 animals per treatment. A one‐way analysis of variance (ANOVA) followed by Tukey post hoc test was used for statistical (**** p < .0001 cisplatin 3 mg/kg vs. saline, *** p < .001 cisplatin 3 mg/kg vs. saline, ** p < .01 cisplatin 3 mg/kg vs. saline, * p < .05 cisplatin 3 mg/kg vs. saline, ### p < .001 cisplatin 3 mg/kg vs. cisplatin 2 mg/kg, ## p < .01 cisplatin 3 mg/kg vs. cisplatin 2 mg/kg, # p < .05 cisplatin 3 mg/kg vs. cisplatin 2 mg/kg).

    Article Snippet: The membranes were blocked with 3% of non‐fat dry milk at room temperature for 1 h and then incubated at 4° overnight with the primary antibodies: TLR4 1:500 (left ventricle), 1:2000 (aorta), 1:1000 (kidney) (Novus Biosciences Cat#NB100‐56566); MyD88 1:500 (left ventricle, kidney), 1:1000 (aorta) (Abcam, Cat#2064); NLRP3 1:750 (left ventricle), 1:1000 (aorta, kidney) (Abcam, Cat#263899); procaspase‐1 1:1000 (left ventricle, aorta, kidney) (Abcam, Cat#286195); NF‐κβ p65 1:1000 (kidney) (Abcam, Cat#16502).

    Techniques: Western Blot, Expressing, Saline